Action de- l’acriflavine sur les levures
APPENDIX In this appendix mass media and buffers needed for the strategies introduced regarding the preceding elements of this part is actually detailed. Media
Slonimski, P
BMM. 1.5 grams malt pull and you will 20 g agar in step 1 L cornmeal extract. Cornmeal pull are taken from 250 g cornmeal incubated during the ten L water from the sixty°C immediately. After that time new supernatant try blocked using multiple layers regarding cheesecloth, therefore the cornmeal is actually discarded. CM average. 0.15% MzP04,0.05% KCI, 0.05% MgS04,1% D- sugar, 0.37% NHEI, 0.2% Pepton, 0.2% yeast pull, step one m& ZnS04,l milligrams/L FeCb Buffers Denaturation bufler: step one.5 M NaCI, 0.5 Meters NaOH Hybridization shield: 50% Formamide (stringent hybridization), 5 X SSPE, 0.5% salt dodecyl sulfate (SDS), 0.step one mg/mL fish jizz DNA. (New stringency from hybridization ide). Mitochondria shield: 0.05 Meters Tris/Cl, 0.01 Meters EDTA, 0.5 Yards sucrose,pH 8.3 Mitochondria rysis boundary: 1% SDS, 0.05 Yards EDTA, 0.02 Meters sodium acetate, pH 5.0; autoclaved Neutralization shield: 2 Meters NaCI, 1 Yards Tris/Cl, pH 5.5 2OX SSC step 1 L includes 175.step three grams NaCl, 88.2 g sodium citrate, pH seven.0 (modified with ten Letter NaOH) 20X SSPE 1 L contains 174 g NaCl, twenty seven.six grams NaH2P04X H20, seven.cuatro grams EDTA, pH adjusted so you can 7.cuatro with 10 N NaCl TE: 10 mM Tris/CI, 1 mM EDTA, pH 8.0 TES: 29 mM Tris/CI, 5 mM EDTA, 50 mM NaCI, pH 8.0 TESISDS: 31 rnM Tris/CI, 5 mM EDTA, fifty mM NaCI, 4% SDS, pH 8.0 TESICsCl: Incorporate step one.1 grams CsCl for every single mL TES and you may to switch refraction list to help you step one.3985
) : 400 mM salt acetate, 800 mM Tns/Cl, forty mM EDTA, pH 8.step three modified having acetic acidic GTC/PME barrier: 5.5 Meters Guanidium isothiocyanate,0.5% sarcosyl, 25 mM salt citrate, 0.step 1 Yards P-mercaptoethanol,pH eight.0 RNA CsCI: 5.7 Meters CsCl, 0.step one Meters EDTA, pH 7.4 References step 1. Lederberg, J. (1952). Mobile genes and you may hereditary symbiosis. Physwl Rev. . dos. Esser, K. (1982). Cryptogumes. School Drive, Cambridge. step three. P., B. Ephrussi (1949). V. Ce systeme de cytochromes de l’ensemble des mutants ‘tiny colonie’. Ann. Inst. Pusteur Purh 77 419. 4. Osiewacz, H. D., J. Hermanns, D. Marcou, Meters. Triffi, K. Esser (1989). Mitochondrial DNA rearrangements are coordinated having a put off amplification of the mobile intron (plDNA) for the an extended-lived mutant off Podospom unserinu. Mutut. Res. 27nine:9. 5 . Rogers, H. J., K. W. Money, C. M.Brasier (1987). Amitochondrial address to possess doublestranded RNA within the unhealthy isolates of the fungi that creates Dutch elm situation. Characteristics 129558. six. Wesolowski, M., H. BuddyGays Fukuhara (1981). Linear mitochondrial desoxyribonucleic acid in the fungus Hunsenulu mrukii. Mol. Cellphone Biol. 1:387. seven. Kovacs, L., J. Lazowska, P. P. Slonimski (1984). A yeast having linear molecules of mitochondrial DNA. Mol. Gen. Genet. 197420. 8. Zimmer, Meters.,G. Luckemann, B. F. Lang, K. Wolf (1984). The fresh new mitochondrial genome of fission fungus Schizosuccharomycespombe. step three. Gene mapping inside filters EFI (CBS 356) and investigation out of hybrids anywhere between challenges EFI and you can ade 7-50 h-. Mol. Gen. Genet. 196473. 9. Hintz, W. E., M. Mohan, J. B. Anderson, P. A. Horgen (1985). The brand new mitochondrial DNA from Agaricus: heterogeneity when you look at the A. bitorquis and homogeneity in the Good. brunnescens. Cur.Genet. 9:127. 10. Hermanns, J., H. D. Osiewacz (1994). Three mitochondrial unassigned unlock discovering frames out of Podosporu unserinu show traces out-of a widespread-kind of RNA polymerase gene. Jizz Genet. . 11. Stahl, U., P. An excellent. Lemke, P. Tudzynski, U. Kuck, K. Esser (1978). Facts to own plasmid particularly DNA into the an effective filamentousfungi, brand new ascomycete Podospora unserinu. MoL Gen. Genet. 162341. twelve. Stahl, U., You. Kuck, P. Tudzynski,K. Esser (1980). Characterization and you may cloning out-of plasmid including DNA of your ascomycete Podosporu unserinu. Mol Gen. Genet. 178 369. 13. Cummings, D. J., L. Belcour, C. Grandchamps (1979). Mitochondria1DNA out-of Podosporu unserinu. 11. Qualities out-of mutant DNA and you will multimeric circular DNA regarding senescent countries. Mol. Gen. Genet. 171
